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Image Search Results
Journal: Science Advances
Article Title: Enhanced TP53 reactivation disrupts MYC transcriptional program and overcomes venetoclax resistance in acute myeloid leukemias
doi: 10.1126/sciadv.adh1436
Figure Lengend Snippet: ( A ) Relative quantitation (RQ) values by quantitative PCR for CDKN1A and MDM2 in OCI-AML3 cells treated with Mil, Sel, or Mil + Sel for the indicated time points. ( B ) Pathway analyses comparing Mil versus Mil + Sel and Sel versus Mil + Sel in RNA-seq in OCI-AML3 cells treated with Mil, Sel, or Mil + Sel for 12 hours. The top up-regulated and down-regulated pathways are indicated by red and blue arrows, respectively. NES, normalized enrichment score. ( C ) Volcano plots [beta score (magnitude) and q values (significance, −log 10 scale)] from differential gene expression profiles in RNA-seq from OCI-AML3 cells DMSO versus Mil (left), Sel (middle), and versus Mil + Sel (right). The top 10 up-regulated TP53 targets and down-regulated MYC targets are indicated with red and blue colors, respectively. The remaining genes are indicated in gray. ( D ) Cell cycle analyses using multiparameter flow cytometry with Ki-67, p21, and cleaved caspase-3 in OCI-AML3 cells treated with Mil, Sel, or Mil + Sel for 24 hours. Caspase-3–negative cells were gated for EdU, Ki-67, and p21 panels. ( E and F ) Change in the percentages of cells in S (E) and M (F) phases in OCI-AML3 cells in indicated treatments. ( G ) The percentages of “p21 high” OCI-AML3 cells [rectangles shown in the third row of (D)] in indicated treatments. ( H ) Change of cell percentages of cleaved caspase-3–positive OCI-AML3 cells [rectangles shown in the fourth row of (D)] in indicated treatments for G 1 (2N) and G 2 -M (4N) phases. A Mil and Sel concentration of 160 nM was used. ** P < 0.01; *** P < 0.001; **** P < 0.0001.
Article Snippet: We used the following antibodies: BCL-X L (141 Pr, clone 54H6; CST, 2764BF),
Techniques: Quantitation Assay, Real-time Polymerase Chain Reaction, RNA Sequencing Assay, Expressing, Flow Cytometry, Concentration Assay